ABSTRACT
Objective To evaluate the diagnostic value of ESAT6 and CFP10 in smear-negative pulmonary tuberculosis.Methods 32 patients of smear-negative pulmonary tuberculosis were selected as the observation group.80 cases of other non-tuberculous respiratory diseases were selected as the control group.ESAT6 and CFP10 polypeptides were used as a stimulating source,the amount of IFN-γsecreted by peripheral blood T lymphocytes was measured by ELISPOT technique.The result was compared with serum anti-tuberculosis antibody (TB-Ab).Results The positive rates of ESAT-6,CFP-10 and combination detection in the observation group were 84.4％,78.1％ and 90.6％ respectively,which were significantly higher than those in the control group,the differences were statistically significant (x2 =71.94,67.43,73.51,all P ＜ 0.01).The sensitivity (90.6％) and negative predictive value (96.1％) of ESAT6,CFP10 combined detection had no statistically significant differences compared with TB-Ab method (x2 =2.20,1.78,all P ＞ 0.05).The specificity (92.5％),positive expected value (82.9％) and the diagnostic accuracy (92.0％) of ESAT6,CFP10 combined detection were significantly higher than those of TB-Ab (60.0％,40.7 ％,62.5 ％),the differences were statistically significant (x2 =11.24,12.07,7.92,all P ＜ 0.01).The positive likelihood ratio of ESAT6,CFP10 combined detection was greater than 10 and negative likelihood ratio was less than 0.1.The possibility of diagnosis was significantly increased.Youden index co-detected by ESAT6 and CFP10 was 0.8,and that detected by TB-Ab was 0.3,indicated that the combined accuracy of ESAT6 and CFP10 was higher than TB-Ab.Conclusion Mycobacterium tuberculosis specific antigen ESAT6,CFP10 can be used as a stimulating source to stimulate effect T lymphocyte to secrete interferon-γ (IFN-γ).IFN-γ can be detected using ELISPOT.Mycobacterium tuberculosis specific antigen ESAT6,CFP10 can be used to quickly assistant the diagnosis of tuberculosis.They have higher sensitivity,specificity and good application value in the auxiliary diagnosis of smear-negative pulmonary tuberculosis.
ABSTRACT
ObjectiveTo discuss the clinical significance of han nationality and kazak hepatitis B virus large surface protein detection.MethodsEnzyme linked immunosorbent assay(ELISA) method was used to examine the HBV-LP、HBV markers and quantitative real-time PCR methods were used to detect the HBV DNA in 270 patients with Hepatitis B.ResultsAmong the 270 cases,there was no significant difference between the levels of HBV DNA and HBV-LP( P ＞ 0.05 )in HBe Ag-positive patients,which was not affected by nationality.Significant difference of positive rate was observed between HBV-LP and HBV DNA( P ＜0.05) in HBe Ag-negative ones,which was not affected by nationality.HBV-Lp expression was significantly correlated with the logarithm of HBV DNA level ( r =0.986,P ＜ 0.05).ConclusionThere was higher coincidence rate between the levels of HBV-LP and HBV DNA in HBeAg--positive patients.The positive rate of HBV-LP was higher than that of HBV DNA in HBe Ag-negative patients.HBV-LP could serve as a reliable marker in the reflection of HBV the replication at protein level,and it was valuable to monitor HBV replication and prognosis of the disease,especially in HBe Ag-negative HBV infected patients.
ABSTRACT
This paper introduces the automatic control system of leechdom management in hospital by computer.It can enhance the managerial level of the medicine and reduce the cost,so the managerial level in hospital can be raised through it.